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The 2 protein bands from forty kDa to 30 kDa have been absent just after 24 h incubation at 45 °C. For the rest of the bands, numerous concentrations of all three proteases retained protein bands equivalent to their unique molar masses (Figure 6a,b). The presence of bands at small molecular weight in fish roe before hydrolysis was sudden. Inside the report by Ghelichi et al. [8], small peaks of a relatively low molecular weight range (three–fourteen kDa) have been noticed in defatted carp roe homogenate, most likely resulting from an autolytic course of action taking place in fish roe and/or the defatting process resulting in the formation of low-molecular-pounds peptides.
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